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Introduction

Introduction to spike-in control

Installation

1. Prerequisites
2. Python Dependencies
3. Install Spiker
4. Upgrade Spiker
5. Uninstall

Usage information

1. Analysis workflow
2. Input and output
3. Spiker.py options
4. split_bam.py options

Walkthrough example

1. Prepare FASTQ files
2. Build bowtie2 index files
3. Map reads to the composite reference genome
4. Calculate scaling factors
5. Run Spiker

Bowtie2 Index Files

1. Bowtie2 index files
2. Index files for Human + Drosophila
3. Index files for Mouse + Drosophila

Test Dataset

1. Test dataset
2. H3K79me2 ChIP-seq data
3. WCE (whole cell extract) data
4. MD5sum

Release history

1. Release history
- 1.1. Version 1.0.0

Liscense

LICENSE

Reference

Reference

Spiker

»
1. Release history
Edit on GitHub

1. Release history

1.1. Version 1.0.0

Initial release

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© Copyright 2021, Liguo Wang. Revision 2e16a156. Last updated on Feb 12, 2021.

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